Research Paper Volume 13, Issue 4 pp 5312—5331

Evaluation of the prognostic values of solute carrier (SLC) family 39 genes for patients with lung adenocarcinoma

SLC39A7 played a vital role in cell survival of lung adenocarcinoma. (A) CERES values of SLC39A7 in various lung adenocarcinoma cell lines, the score less than -1 means the gene is important for cell survival. (B) After 48 h transfected with control siRNA or siRNA-SLC39A7, A549 cells were harvested. The lysates were undergone for detecting SLC39A7 by Western blotting. (C) After seeding, A549 cells were transfected with control siRNA or siRNA-SLC39A7. The CCK-8 assay was performed at 0.5, 1.5, 2.5 and 3.5 days after transfection. The cell viability of siRNA-SLC39A7 treatment was normalized to control group. (D) A549 cells were transfected with control siRNA or siRNA-SLC39A7. Then the quantitative cells were placed and cultured for 14 day, and stained with crystal violet-glutaraldehyde solution. All experiments performed in triplicate, and the results are calculated using mean ± standard deviation. One-way ANOVA or Student t test was carried out for evaluating the statistical significance between groups. *** P

Figure 4. SLC39A7 played a vital role in cell survival of lung adenocarcinoma. (A) CERES values of SLC39A7 in various lung adenocarcinoma cell lines, the score less than -1 means the gene is important for cell survival. (B) After 48 h transfected with control siRNA or siRNA-SLC39A7, A549 cells were harvested. The lysates were undergone for detecting SLC39A7 by Western blotting. (C) After seeding, A549 cells were transfected with control siRNA or siRNA-SLC39A7. The CCK-8 assay was performed at 0.5, 1.5, 2.5 and 3.5 days after transfection. The cell viability of siRNA-SLC39A7 treatment was normalized to control group. (D) A549 cells were transfected with control siRNA or siRNA-SLC39A7. Then the quantitative cells were placed and cultured for 14 day, and stained with crystal violet-glutaraldehyde solution. All experiments performed in triplicate, and the results are calculated using mean ± standard deviation. One-way ANOVA or Student t test was carried out for evaluating the statistical significance between groups. *** P<0.001. SLC39A, solute carrier family 39.