Figure 1. Correlation between HT Q-FISH and PCR and TRF techniques for telomere length measurements. (A) Correlation of telomere length measured by TRF, qPCR and HT Q-FISH in Peripheral blood mononuclear cell (PMBC) samples from 89 individuals. (B, C) Linear regression analysis was used to determine the rate of telomere shortening (B) and the rate of the increase of short telomeres (<3kb) (C) per year in PMBCs. The telomere length data in (B, C) correspond to HT-qFish analysis. The Pearson correlation coefficient and linear regression equation were determined using GraphPad Software.