Research Paper Volume 13, Issue 1 pp 89—103

Further assessment of Salvia haenkei as an innovative strategy to counteract skin photo-aging and restore the barrier integrity

Effect of Salvia haenkei extract 0.01-0.1-1-10 μg/ml on ROS generation in HaCaT cells in basal and UVB-stimulated conditions (30 KJ/m2). ROS were measured after 3-24 hours of incubation with SH under basal condition (A) and after UVB exposure (B, C). Figure (C) represents the effect of UVB stimulation of untreated cells, while figure (B) represents the effect of UVB stimulation of treated cells. NAC 5 mM is used as positive control. Data are expressed as mean ± SEM of fluorescence intensity (FI) of treated cells related to control. n = 3-4 experiments. *pppvs control.

Figure 3. Effect of Salvia haenkei extract 0.01-0.1-1-10 μg/ml on ROS generation in HaCaT cells in basal and UVB-stimulated conditions (30 KJ/m2). ROS were measured after 3-24 hours of incubation with SH under basal condition (A) and after UVB exposure (B, C). Figure (C) represents the effect of UVB stimulation of untreated cells, while figure (B) represents the effect of UVB stimulation of treated cells. NAC 5 mM is used as positive control. Data are expressed as mean ± SEM of fluorescence intensity (FI) of treated cells related to control. n = 3-4 experiments. *p<0.05, **p<0.01, ***p<0.001treatment vs control.