Research Paper Volume 13, Issue 4 pp 5369—5382

Inhibiting TLR4 signaling by linarin for preventing inflammatory response in osteoarthritis

Influence of Linarin in LPS-medicated ECM degeneration in human chondrocytes. (A) The level of Collagen II, aggrecan, MMP13 and ADAMTS-5 in chondrocytes treated as above were visualized by ELISA. (B) The representative collagen II and MMP13 was detected by the immunofluorescence combined with DAPI staining for nuclei (scale bar: 50μm or 30μm). The data in the figures represent the averages ± S.D. Significant differences among different groups are indicated as ##P P P

Figure 3. Influence of Linarin in LPS-medicated ECM degeneration in human chondrocytes. (A) The level of Collagen II, aggrecan, MMP13 and ADAMTS-5 in chondrocytes treated as above were visualized by ELISA. (B) The representative collagen II and MMP13 was detected by the immunofluorescence combined with DAPI staining for nuclei (scale bar: 50μm or 30μm). The data in the figures represent the averages ± S.D. Significant differences among different groups are indicated as ##P < 0.01, vs. control group; *P < 0.05, **P < 0.01 vs. LPS alone treatment group, n=5.