Figure 2. Comparing with controls, LECs aging was increasing in ARCC. (A). The number of SA-β-gal-positive cells increased in LECs of ARCC compared with controls. The senescence of LECs in ARCC was significantly increasing. Scale bar: 50μm. (B). The expression levels of proteins in ARCC and controls were detected by western blot, expression of aging related protein p53 and p21 were higher than controls. **p <0.01. (C). Immunofluorescence showed that p53 and p21 were co-located in the nucleus, while the upstream protein of ATM was located in the cytoplasm in ARCC. with the nuclei staining with DAPI (blue). Scale bar: 50 μm.