Research Paper Volume 13, Issue 4 pp 5383—5402

CircMRE11A_013 binds to UBXN1 and integrates ATM activation enhancing lens epithelial cells senescence in age-related cataract

Over-expression of circMRE11A inhibited cell viability and cell cycle of SRA01/04 cell lines in vitro. (A). The qRT-PCR results showed that cells transfected with the construct expressed higher level of circMRE11A than that in the vector controls. *p B). Transfection with circMRE11A did not affect levels of MRE11A mRNA. (C). CCK-8 showed that the cell viability was decreased in the over-circMRE11A group compared with controls. *p D). Flow cytometry demonstrated that over-circMRE11A induced G1/S arrest in the cell lines. (E). The expression of ATM-S1981p was higher in the over-circMRE11A group compared with controls. Meanwhile, over-circMRE11A did not affect the levels of proteins MRE11A, ATM, p53 and p21. Tubulin and GAPDH levels were measured as internal controls. *p

Figure 5. Over-expression of circMRE11A inhibited cell viability and cell cycle of SRA01/04 cell lines in vitro. (A). The qRT-PCR results showed that cells transfected with the construct expressed higher level of circMRE11A than that in the vector controls. *p <0.05. (B). Transfection with circMRE11A did not affect levels of MRE11A mRNA. (C). CCK-8 showed that the cell viability was decreased in the over-circMRE11A group compared with controls. *p <0.05. (D). Flow cytometry demonstrated that over-circMRE11A induced G1/S arrest in the cell lines. (E). The expression of ATM-S1981p was higher in the over-circMRE11A group compared with controls. Meanwhile, over-circMRE11A did not affect the levels of proteins MRE11A, ATM, p53 and p21. Tubulin and GAPDH levels were measured as internal controls. *p <0.05.