Figure 4. Axin1 is a target of miR-3574, and Axin1 could be inhibited by miR-3574 in H9c2 cells. (A) The potential binding site between miR-3574 and the 3’-UTR of Axin1. (B) Luciferase reporter assay was conducted by co-transfection of luciferase reporter containing 3’-UTR of Axin1 with miR-3574 mimic into 293T cells. (C–E) H9c2 cells were transfected with miR-3574 mimics or corresponding control. Expressions of Axin1 were examined by western blot analysis and RT-qPCR, respectively. NC: negative control. Data are represented as the mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01.