Figure 5. Upregulation of miR-3574 inhibits IH-induced H9c2 cardiomyocyte injury via Axin1. miR-3574 mimic, pcDNA3.1-Axin1 plasmid, and scrambled control were transfected into H9c2 cells. Cells without transfection were served as control. (A) Cell viability. (B, C) Western blot assays of Caspase-3, Bax, Bcl-2, and Axin1 protein. NC: negative control. IH: intermittent hypoxia. Data are represented as the mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01.