Figure 4. Effect of melatonin on apoptosis in EB1-exposed embryos. (A) blastocyst cell apoptosis status (× 200). (B) average apoptotic cell number per blastocyst. (C) blastocyst cell apoptotic rate. The number of blastocysts for apoptosis detection in the CON, EB1, MT or ME group was 29, 16, 32 or 25, respectively. CON, the control group. EB1, embryos treated with EB1. MT, embryos treated with melatonin. ME, embryos treated with both melatonin and EB1. a-dValues for a certain group in columns with different superscripts differ significantly (p < 0.05).