Figure 2. Knockdown of CRT significantly inhibits cell proliferation in vitro. (A) CRT expression following knockdown was confirmed by western blot assay. (B, C) CCK-8 assay was performed to analyze the proliferation of GBC-SD and NOZ cells. (D) Cell colony formation ability was detected by colony formation assay.*P<0.05 vs. negative control. CRT, calreticulin; GBC, gallbladder cancer.