Figure 4. Knockdown of CRT significantly inhibits cell migration in vitro. (A) Cell migratory ability was detected by wound healing assay. (B) Cell migratory ability was detected by Transwell assay. Scale bars =200um. (C) Western blot assay revealed that knockdown of CRT inhibited the expression of p-Akt and MMP-9 in GBC-SD and NOZ cells.*P<0.05 vs. negative control. p-, phosphorylated; CRT, calreticulin; GBC, gallbladder cancer.