Research Paper Volume 13, Issue 4 pp 5698—5717

Identification of novel candidate biomarkers for pancreatic adenocarcinoma based on TCGA cohort

MiR-16 expression was dramatically decreased in PAAD and positively correlated with prognosis of PAAD patients. TG inhibited PANC-1 cell viability via modulating PI3K pathway. (A) Expression level of miR-16 between PAAD tissue samples and normal pancreatic tissues. (B) Overall survival curve of miR-16 in PAAD patients was plotted using Kaplan-Meier analysis. (C) The expression level of miR-16 in HPDE6C7, PANC-1, SW1990, and BXPC3. (D, E) Cell proliferative ability was measured using CCK-8 assay after TG treatment with different concentrations or times. (F) Cell viability was detected followed by TG or LY294002 induction. (G) Western blot was performed to examine the expression of OSMR, ITGB1, ITGB5, p-PI3K and PI3K in PANC-1 cells. PAAD, pancreatic adenocarcinoma; TG, thapsigargin.

Figure 7. MiR-16 expression was dramatically decreased in PAAD and positively correlated with prognosis of PAAD patients. TG inhibited PANC-1 cell viability via modulating PI3K pathway. (A) Expression level of miR-16 between PAAD tissue samples and normal pancreatic tissues. (B) Overall survival curve of miR-16 in PAAD patients was plotted using Kaplan-Meier analysis. (C) The expression level of miR-16 in HPDE6C7, PANC-1, SW1990, and BXPC3. (D, E) Cell proliferative ability was measured using CCK-8 assay after TG treatment with different concentrations or times. (F) Cell viability was detected followed by TG or LY294002 induction. (G) Western blot was performed to examine the expression of OSMR, ITGB1, ITGB5, p-PI3K and PI3K in PANC-1 cells. PAAD, pancreatic adenocarcinoma; TG, thapsigargin.