Research Paper Volume 13, Issue 4 pp 5804—5823

Macrophage migration inhibitory factor activates the inflammatory response in joint capsule fibroblasts following post-traumatic joint contracture

Expression profiling and inflammation-related factors identification of integrated DEGs analysis. (A) Heat map of integrated DEGs in the top 30 most significantly enriched groups. Red represents upregulation. Blue represents downregulation. (B–D) qRT-PCR analysis of the expression of Pla2g2a, Angptl4, and Sgpp2 in JFs after treatment with 0–2.5 μg/mL recombinant MIF for 24 h (B), treatment with 2 μg/mL MIF combined with 50 μ5M 4-IPP for 24 h (C), or treatment with siRNA2 for 48 h, followed by stimulation with 2 μg/mL MIF for 24 h (D). Error bars represent standard deviation. *P

Figure 10. Expression profiling and inflammation-related factors identification of integrated DEGs analysis. (A) Heat map of integrated DEGs in the top 30 most significantly enriched groups. Red represents upregulation. Blue represents downregulation. (BD) qRT-PCR analysis of the expression of Pla2g2a, Angptl4, and Sgpp2 in JFs after treatment with 0–2.5 μg/mL recombinant MIF for 24 h (B), treatment with 2 μg/mL MIF combined with 50 μ5M 4-IPP for 24 h (C), or treatment with siRNA2 for 48 h, followed by stimulation with 2 μg/mL MIF for 24 h (D). Error bars represent standard deviation. *P <0.05 compared with the 0 μg/mL or control group.