Figure 5. MIF activated inflammation in JFs. (A) Expression of Tnf-α, Il-1β, and Il-6 was assessed via qRT-PCR following JFs treatment with 0–2.5 μg/mL recombinant MIF for 24 h. (B–D) Levels of TNF-α (B), IL-1β (C), and IL-6 (D) in cell supernatants and lysates were measured using ELISA. (E) JFs were treated with 2 μg/mL recombinant MIF combined with 50 μM 4-IPP for 24 h, qRT-PCR evaluated Tnf-α, Il-1β, and Il-6 expression. Error bars represent standard deviation. *P <0.05 compared with the 0 μg/mL or DMSO group. #P <0.05 compared with the MIF group.