Figure 6. MIF promoted the proliferation and migration of JFs in vitro. (A, C) JFs were treated with 0–2.5 μg/mL recombinant MIF for 24 h before testing cell proliferation using an EdU assay (A) and cell migration via a Transwell assay (C). (B, D) JFs were treated with 2 μg/mL recombinant MIF combined with 50 μM 4-IPP for 24 h followed by EdU assay (B) and Transwell assay (D). Scale bar, 50 μm. Error bars represent standard deviation. *P <0.05 compared with the 0 μg/mL or DMSO group. #P <0.05 compared with the MIF group.