Research Paper Volume 13, Issue 4 pp 5858—5874

GABARAP suppresses EMT and breast cancer progression via the AKT/mTOR signaling pathway

GABARAP is downregulated in breast cancer specimens and cell lines. (A) Expression profile of GABARAP in primary breast cancer tissues (n = 1097) and normal breast tissues (n = 114) (TCGA). (B) Expression of GABARAP in BRCA based on breast cancer subclasses (TCGA). (C) Expression of GABARAP in BRCA based on individual cancer stages (TCGA). (D) Overall survival of breast cancer patients according to GABARAP expression (TCGA). (E) Representative images of GABARAP immunohistochemical staining. (magnification, 100× and 400×). (F) Statistical significance of ‘positive’ or ‘negative’ GABARAP staining in 87 cases of IDC, 48 cases of DCIS and 24 cases of normal breast tissue. (G) Western blotting analysis of GABARAP expression in 8 human breast cancer cell lines and non-transformed MCF-10A cells. (H) Statistical significance of GABARAP expression in 8 human breast cancer cell lines and non-transformed MCF-10A cells. (*P P P P

Figure 1. GABARAP is downregulated in breast cancer specimens and cell lines. (A) Expression profile of GABARAP in primary breast cancer tissues (n = 1097) and normal breast tissues (n = 114) (TCGA). (B) Expression of GABARAP in BRCA based on breast cancer subclasses (TCGA). (C) Expression of GABARAP in BRCA based on individual cancer stages (TCGA). (D) Overall survival of breast cancer patients according to GABARAP expression (TCGA). (E) Representative images of GABARAP immunohistochemical staining. (magnification, 100× and 400×). (F) Statistical significance of ‘positive’ or ‘negative’ GABARAP staining in 87 cases of IDC, 48 cases of DCIS and 24 cases of normal breast tissue. (G) Western blotting analysis of GABARAP expression in 8 human breast cancer cell lines and non-transformed MCF-10A cells. (H) Statistical significance of GABARAP expression in 8 human breast cancer cell lines and non-transformed MCF-10A cells. (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. TCGA = The Cancer Genome Atlas).