Figure 3. CircEPSTI1 acts as a sponge of miRNAs for miR-375, miR-409-3P and miR-515-5p. (A) The localization of circEPSTI1 in cell was examined in HeLa cells. (B) Schematic diagram of the predicted miR-375, miR-409-3P and miR-515-5p sites in the circEPSTI1. (C) QRT-PCR detected the miR-375, miR-409-3P and miR-515-5p levels in cervical cancer cell lines (HcerEpic, HeLa and CaSki). (D) MS2-based RIP assay in cervical cancer cells transfected with MS2bs-Rluc vector (control), MS2bs-circEPSTI1 vector or MS2bs-circEPSTI1mt vector. (E) Knockdown circEPSTI1 significantly upregulated the expression level of miR-375/409-3P/515-5p. **, P < 0.01 compared to the negative control group.