Research Paper Volume 13, Issue 5 pp 6662—6680

The novel roles of virus infection-associated gene CDKN1A in chemoresistance and immune infiltration of glioblastoma

CDKN1A was involved in TMZ resistance of glioma cells. (A) Western blot for phospho-Akt (Ser-473), Akt in U87, U87-R T98G, and T98G-R cells. (B) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R or T98G-R cells treated with DMSO and the specific inhibitor to AKT MK2206. (C) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R or T98G-R cells treated with siCtrl and siCDKN1A. (D) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. (E) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in T98G-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. (F, G) Colony formation assay of U87-R or T98G-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. The results were presented as means ± SD (n = 3 for each panel). Statistical significance was concluded at *P

Figure 6. CDKN1A was involved in TMZ resistance of glioma cells. (A) Western blot for phospho-Akt (Ser-473), Akt in U87, U87-R T98G, and T98G-R cells. (B) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R or T98G-R cells treated with DMSO and the specific inhibitor to AKT MK2206. (C) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R or T98G-R cells treated with siCtrl and siCDKN1A. (D) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in U87-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. (E) Western blot for phospho-Akt (Ser-473), Akt, phospho-CDKN1A, and CDKN1A in T98G-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. (F, G) Colony formation assay of U87-R or T98G-R cells treated with DMSO, MK2206, siCDKN1A and MK2206 plus siCDKN1A. The results were presented as means ± SD (n = 3 for each panel). Statistical significance was concluded at *P < 0.05, **P < 0.01.