Research Paper Volume 13, Issue 4 pp 5967—5985

Transfer of lncRNA UCA1 by hUCMSCs-derived exosomes protects against hypoxia/reoxygenation injury through impairing miR-143-targeted degradation of Bcl-2

The hUCMSC-ex protects CMECs against autophagy following H/R injury. (A) Transmission electron microscope observation of autophagosomes; (B) Fluorescence localization of LC3 in CMECs under exposure to H/R injury. LC3 was labeled as green fluorescence, nucleus were labeled by DAPI and CMEC marker CD31 was labeled as red fluorescence. (C) LC3-II/LC3-I, Beclin-1 and p62 expression in CMECs under exposure to H/R injury measured by Western blot assay. *p vs. the control group; #p vs. the H/R or I/R group. All experiments were repeated 3 times. Data in panels (B and C) were analyzed with one-way ANOVA and Tukey’s multiple comparisons test.

Figure 4. The hUCMSC-ex protects CMECs against autophagy following H/R injury. (A) Transmission electron microscope observation of autophagosomes; (B) Fluorescence localization of LC3 in CMECs under exposure to H/R injury. LC3 was labeled as green fluorescence, nucleus were labeled by DAPI and CMEC marker CD31 was labeled as red fluorescence. (C) LC3-II/LC3-I, Beclin-1 and p62 expression in CMECs under exposure to H/R injury measured by Western blot assay. *p < 0.05, vs. the control group; #p < 0.05, vs. the H/R or I/R group. All experiments were repeated 3 times. Data in panels (B and C) were analyzed with one-way ANOVA and Tukey’s multiple comparisons test.