Research Paper Volume 13, Issue 4 pp 5967—5985

Transfer of lncRNA UCA1 by hUCMSCs-derived exosomes protects against hypoxia/reoxygenation injury through impairing miR-143-targeted degradation of Bcl-2

LncRNA UCA1 silencing in hUCMSC-ex facilitates H/R injury in CMECs. (A) LncRNA UCA1 expression in CMECs determined by RT-qPCR; (B) Viability of CMECs detected by CCK-8. (C) Invasion of CMECs assessed by Transwell assay. (D) Migration of CMECs evaluated by scratch test after co-culture with hUCMSC-ex. (E) The number of TUNEL-positive CMECs. (F) Apoptotic rate of CMECs assessed by flow cytometry. (G) Expression of apoptosis-related proteins Cleaved caspase3 and Bax in CMECs measured by Western blot assay. (H) Tube formation in CMECs. *p vs. the control or hUCMSCs-ex group; #p vs. the H/R group. All experiments were repeated 3 times. Data in panel (G) were analyzed with two-way ANOVA, and data in other panels were analyzed with one-way ANOVA, followed by Tukey’s multiple comparisons test.

Figure 5. LncRNA UCA1 silencing in hUCMSC-ex facilitates H/R injury in CMECs. (A) LncRNA UCA1 expression in CMECs determined by RT-qPCR; (B) Viability of CMECs detected by CCK-8. (C) Invasion of CMECs assessed by Transwell assay. (D) Migration of CMECs evaluated by scratch test after co-culture with hUCMSC-ex. (E) The number of TUNEL-positive CMECs. (F) Apoptotic rate of CMECs assessed by flow cytometry. (G) Expression of apoptosis-related proteins Cleaved caspase3 and Bax in CMECs measured by Western blot assay. (H) Tube formation in CMECs. *p < 0.05, vs. the control or hUCMSCs-ex group; #p < 0.05, vs. the H/R group. All experiments were repeated 3 times. Data in panel (G) were analyzed with two-way ANOVA, and data in other panels were analyzed with one-way ANOVA, followed by Tukey’s multiple comparisons test.