Research Paper Volume 13, Issue 4 pp 5986—6009

Overexpression of hsa_circ_0002874 promotes resistance of non-small cell lung cancer to paclitaxel by modulating miR-1273f/MDM2/p53 pathway

Screening of circRNA and determination of the downstream miRNA. (A, B) Screening for circRNA with the greatest change after PTX administration by qPCR. (C) Confirmation of the hsa

Figure 1. Screening of circRNA and determination of the downstream miRNA. (A, B) Screening for circRNA with the greatest change after PTX administration by qPCR. (C) Confirmation of the hsa_circ_0002874 expression at different time points after PTX administration by qPCR. (D) Prediction of target miRNAs for hsa_circ_0002874 by circMir 1.0, RegRNA 2.0 and MirTrap software. (E) Changes in the four predicted miRNAs expression after PTX administration by qPCR. (F) Changes in target genes expression for 4 predicted miRNAs after PTX administration by qPCR. (G) Prediction of the binding stability of hsa_circ_0002874 and miR1273f by Reg RNA2.0 website. (H) Prediction of the binding stability of miR1273f and MDM2 mRNA by TargetScan Human 7.2 website. *p <0.05, **p <0.01, ***p <0.001, n=3 in each group.