Research Paper Volume 13, Issue 4 pp 6010—6024

Exosomal long non-coding RNA LINC00662 promotes non-small cell lung cancer progression by miR-320d/E2F1 axis

Exosomal LINC00662 contributes to the tumor growth of NSCLC in vivo. (A–D) The effect of exosomal LINC00662 on tumor growth of NSCLC cells in vivo was analyzed by nude mice tumorigenicity assay. The A549 cells were treated with LINC00662 overexpression vector or LINC00662 overexpression exosome. (A) Representative images of dissected tumors from nude mice were presented. (B) The average tumor volume was calculated and shown. (C) The average tumor weight was calculated and shown. (D, E) The protein expression levels of E2F1 and β-actin were examined by Western blot analysis in the tumor tissues. Data are presented as mean ± SD. Statistic significant differences were indicated: * P P

Figure 7. Exosomal LINC00662 contributes to the tumor growth of NSCLC in vivo. (AD) The effect of exosomal LINC00662 on tumor growth of NSCLC cells in vivo was analyzed by nude mice tumorigenicity assay. The A549 cells were treated with LINC00662 overexpression vector or LINC00662 overexpression exosome. (A) Representative images of dissected tumors from nude mice were presented. (B) The average tumor volume was calculated and shown. (C) The average tumor weight was calculated and shown. (D, E) The protein expression levels of E2F1 and β-actin were examined by Western blot analysis in the tumor tissues. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05, ** P < 0.01.