Figure 3. Peripheral indoxyl sulfate treatment increased its blood and CNS distribution. Unilateral nephrectomized mice were intraperitoneally injected with indoxyl sulfate (IS, 0 and 100 mg/kg) and the indoxyl sulfate-injected mice were orally given with AST-120 (0 and 400 mg/kg) for 7 weeks. The blood (A), prefrontal cortical tissue (B), and CSF (C) were isolated and subjected to the measurement of indoxyl sulfate. *p < 0.05 vs. control group, and #p < 0.05 vs. indoxyl sulfate alone (IS) group, n = 8.