Research Paper Volume 13, Issue 5 pp 6752—6764

Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury

EVs-encapsulated miR-23a is enriched in cultured DRG neurons following capsaicin induction. (A) representative Western blots of TSG101, Flotillin-1, and MFG-E8 proteins in DRG neurons treated with capsaicin, normalized to β-actin; (B) quantitation of the protein levels of TSG101, Flotillin-1, and MFG-E8 in DRG neurons treated with capsaicin measured using Western blot analysis, normalized to β-actin; (C) EVs detected by NanoSight; (D) miR-23a expression in DRG neurons treated with capsaicin determined using RT-qPCR, normalized to U6. Values obtained from three independent experiments are expressed as mean ± SD and analyzed by unpaired t-test. * indicates p

Figure 2. EVs-encapsulated miR-23a is enriched in cultured DRG neurons following capsaicin induction. (A) representative Western blots of TSG101, Flotillin-1, and MFG-E8 proteins in DRG neurons treated with capsaicin, normalized to β-actin; (B) quantitation of the protein levels of TSG101, Flotillin-1, and MFG-E8 in DRG neurons treated with capsaicin measured using Western blot analysis, normalized to β-actin; (C) EVs detected by NanoSight; (D) miR-23a expression in DRG neurons treated with capsaicin determined using RT-qPCR, normalized to U6. Values obtained from three independent experiments are expressed as mean ± SD and analyzed by unpaired t-test. * indicates p < 0.05. Cell experiment was independently repeated for three times.