Research Paper Volume 13, Issue 5 pp 6765—6781

Growth hormone ameliorates the age-associated depletion of ovarian reserve and decline of oocyte quality via inhibiting the activation of Fos and Jun signaling

The GH levels and oocyte quality were declined in aged mice. (A) The GH levels in the peripheral blood were measured in young (n = 6) and aged (n = 6) mice. (B) Micrographs of young and aged WT mouse ovaries (Scale bar, 1 mm) and HE staining of these ovaries (Scale bar, 100μm, 500 μm). (C) Follicle counts in 6-week (n = 3) and 10-month (n = 3) old WT mice. (D) Chromosomes misalignment and spindle defects (arrowheads) in aged oocytes. The oocytes were stained with α-tubulin (green) and propidium iodide (PI) (red) respectively. Scale bar, 50 μm. (E) Left: The rate of GVBD and Pb1 extrusion were recorded after 4 h and 14 h of culture in M2 medium respectively. Right: Percentages of oocytes with spindle defects in young (n = 97) and aged mice (n = 104). (F) Representative images of MII oocytes collected from young (n = 76) and aged (n = 19) mice and IVF outcomes from these two groups. Black arrowheads point to abnormal oocytes. Scale bar, 100 μm, 400 μm. (G) Quantification of MII oocytes, 2-cell embryo and blastocyst from young and aged mice. Data are presented as mean ± SD. *P P P

Figure 1. The GH levels and oocyte quality were declined in aged mice. (A) The GH levels in the peripheral blood were measured in young (n = 6) and aged (n = 6) mice. (B) Micrographs of young and aged WT mouse ovaries (Scale bar, 1 mm) and HE staining of these ovaries (Scale bar, 100μm, 500 μm). (C) Follicle counts in 6-week (n = 3) and 10-month (n = 3) old WT mice. (D) Chromosomes misalignment and spindle defects (arrowheads) in aged oocytes. The oocytes were stained with α-tubulin (green) and propidium iodide (PI) (red) respectively. Scale bar, 50 μm. (E) Left: The rate of GVBD and Pb1 extrusion were recorded after 4 h and 14 h of culture in M2 medium respectively. Right: Percentages of oocytes with spindle defects in young (n = 97) and aged mice (n = 104). (F) Representative images of MII oocytes collected from young (n = 76) and aged (n = 19) mice and IVF outcomes from these two groups. Black arrowheads point to abnormal oocytes. Scale bar, 100 μm, 400 μm. (G) Quantification of MII oocytes, 2-cell embryo and blastocyst from young and aged mice. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0005.