Research Paper Volume 13, Issue 5 pp 6765—6781

Growth hormone ameliorates the age-associated depletion of ovarian reserve and decline of oocyte quality via inhibiting the activation of Fos and Jun signaling

Effects of GH treatment in vitro on the meiotic progress of aged oocytes. (A) Representative images of oocytes after 14 hours cultured in M2 medium (n = 87) and GH treated medium (n = 88). Black arrowheads point to oocytes that fail to extrude a polar body or fail to survive. Scale bar, 100 μm. (B) The rate of GVBD and Pb1 extrusion in these two groups respectively. (C) Left: Spindle morphologies in control and GH group. Oocytes were stained with α-tubulin (green) and hoechst (blue). Scale bar, 50 μm. Right: Percentages of oocytes with spindle defects in control (n = 51) and GH group (n = 68). Data are presented as mean ± SD. **P

Figure 2. Effects of GH treatment in vitro on the meiotic progress of aged oocytes. (A) Representative images of oocytes after 14 hours cultured in M2 medium (n = 87) and GH treated medium (n = 88). Black arrowheads point to oocytes that fail to extrude a polar body or fail to survive. Scale bar, 100 μm. (B) The rate of GVBD and Pb1 extrusion in these two groups respectively. (C) Left: Spindle morphologies in control and GH group. Oocytes were stained with α-tubulin (green) and hoechst (blue). Scale bar, 50 μm. Right: Percentages of oocytes with spindle defects in control (n = 51) and GH group (n = 68). Data are presented as mean ± SD. **P < 0.01.