Research Paper Volume 13, Issue 5 pp 6765—6781

Growth hormone ameliorates the age-associated depletion of ovarian reserve and decline of oocyte quality via inhibiting the activation of Fos and Jun signaling

Effect of GH treatment on the FOS/JUN pathway. (A) The expression of GHR in GH-treated and NS-treated oocytes. (B) The expression of Fos, Fosb, Jun, Junb, Jund, Lmnb2 and Ctsb in GH-treated and NS-treated oocytes according to the sequencing data. (C) Results of single oocyte QPCR in the two groups (n ≥ 10). (D) Immunostaining for Fos, Jun, Fosb and Junb in ovaries from the NS and GH groups. Brown represents positive staining. Data are presented as mean ± SD. *P

Figure 7. Effect of GH treatment on the FOS/JUN pathway. (A) The expression of GHR in GH-treated and NS-treated oocytes. (B) The expression of Fos, Fosb, Jun, Junb, Jund, Lmnb2 and Ctsb in GH-treated and NS-treated oocytes according to the sequencing data. (C) Results of single oocyte QPCR in the two groups (n ≥ 10). (D) Immunostaining for Fos, Jun, Fosb and Junb in ovaries from the NS and GH groups. Brown represents positive staining. Data are presented as mean ± SD. *P < 0.05.