Figure 3. SIRT6 overexpression enhances telomerase activity and inhibits fibrosis in ligamentum flavum cells. SIRT6 or control lentiviral vectors were used to transduce LFH cells for 48 h, after which western blotting was used to assess SIRT6 levels in these cells (A, B). α-tubulin was used for normalization. Uninfected LFH cells and LFN cells were included as controls. (C) Telomerase activity in ligamentum flavum cells transduced as indicated was assessed based upon optical density. (D, E) TGF-β1, α-SMA, and collagen I protein levels were analyzed by western blotting. β-actin was used as a loading control. Data are means ± SD of three replicates. *p<0.05, **p<0.01 vs. LFN. #p<0.05 vs. LFH control.