Research Paper Volume 13, Issue 4 pp 6025—6040

SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients

The impact of SIRT6 overexpression on telomerase activity and fibrosis-related protein expression is reversed by hTERT knockdown. LFH cells were infected using lentiviral vectors encoding SIRT6 (pSIRT6), an hTERT-specific shRNA (sh-hTERT), or a control shRNA, after which Western blotting was used to assess the expression of SIRT6 in these cells (A, B), with α-tubulin being used for normalization. (C) Telomerase activity in ligamentum flavum cells treated as indicated was assessed based upon optical density. (D, E) TGF-β1, α-SMA, and collagen I protein levels were analyzed by western blotting, with β-actin being used for normalization. Data are means ± SD of three replicates. *pp

Figure 4. The impact of SIRT6 overexpression on telomerase activity and fibrosis-related protein expression is reversed by hTERT knockdown. LFH cells were infected using lentiviral vectors encoding SIRT6 (pSIRT6), an hTERT-specific shRNA (sh-hTERT), or a control shRNA, after which Western blotting was used to assess the expression of SIRT6 in these cells (A, B), with α-tubulin being used for normalization. (C) Telomerase activity in ligamentum flavum cells treated as indicated was assessed based upon optical density. (D, E) TGF-β1, α-SMA, and collagen I protein levels were analyzed by western blotting, with β-actin being used for normalization. Data are means ± SD of three replicates. *p<0.05, vs. non-infection control. ##p<0.01 vs. pSIRT6- and control shRNA-transduced cells (pSIRT6+shRNA control).