Research Paper Volume 13, Issue 4 pp 6025—6040

SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients

SIRT6 inhibits LFH cell senescence via promoting telomerase activity. LFH cells were transduced for 48 h using lentiviruses encoding pSIRT6, sh-hTERT, or a control shRNA, after which senescence-associated β-galactosidase (SA-β-gal) staining of these cells was conducted. Uninfected cells were used as controls. (A) Representative bright-field images of SA-β-Gal stained cells. Scale bar = 100 μm. (B) SA-β-gal positive cell percentages. mRNA levels of the senescence-related genes p16 (C), MMP3 (D), and Long interspersed element 1 (L1, E) were quantified via qRT-PCR, with GAPDH being used as a normalization control. (F) Telomerase activity in ligamentum flavum cells treated as indicated was assessed based upon optical density. Data are means ± SD of three replicates. *pppp

Figure 5. SIRT6 inhibits LFH cell senescence via promoting telomerase activity. LFH cells were transduced for 48 h using lentiviruses encoding pSIRT6, sh-hTERT, or a control shRNA, after which senescence-associated β-galactosidase (SA-β-gal) staining of these cells was conducted. Uninfected cells were used as controls. (A) Representative bright-field images of SA-β-Gal stained cells. Scale bar = 100 μm. (B) SA-β-gal positive cell percentages. mRNA levels of the senescence-related genes p16 (C), MMP3 (D), and Long interspersed element 1 (L1, E) were quantified via qRT-PCR, with GAPDH being used as a normalization control. (F) Telomerase activity in ligamentum flavum cells treated as indicated was assessed based upon optical density. Data are means ± SD of three replicates. *p<0.05, **p<0.01, ***p<0.001 vs. LFN. #p<0.05 vs. LFH control.