Research Paper Volume 13, Issue 4 pp 6025—6040

Figure 5. SIRT6 inhibits LFH cell senescence via promoting telomerase activity. LFH cells were transduced for 48 h using lentiviruses encoding pSIRT6, sh-hTERT, or a control shRNA, after which senescence-associated β-galactosidase (SA-β-gal) staining of these cells was conducted. Uninfected cells were used as controls. (A) Representative bright-field images of SA-β-Gal stained cells. Scale bar = 100 μm. (B) SA-β-gal positive cell percentages. mRNA levels of the senescence-related genes p16 (C), MMP3 (D), and Long interspersed element 1 (L1, E) were quantified via qRT-PCR, with GAPDH being used as a normalization control. (F) Telomerase activity in ligamentum flavum cells treated as indicated was assessed based upon optical density. Data are means ± SD of three replicates. *p<0.05, **p<0.01, ***p<0.001 vs. LFN. #p<0.05 vs. LFH control.