Research Paper Volume 13, Issue 4 pp 6025—6040

SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients

SIRT6 protects LFH cells from DNA damage and telomere dysfunction via activation of telomerase activity. (A) Representative images of LF cells stained for γH2AX (red) and TRF-1 (green) and counterstained with DAPI (blue). Scale bar = 5 μm. The mean number of γH2AX foci (B), TRF-1 (C) and telomere dysfunction-induced foci (TIFs, D) in cells were quantified using ImageJ. Data are means ± SD of three replicates. *ppp

Figure 6. SIRT6 protects LFH cells from DNA damage and telomere dysfunction via activation of telomerase activity. (A) Representative images of LF cells stained for γH2AX (red) and TRF-1 (green) and counterstained with DAPI (blue). Scale bar = 5 μm. The mean number of γH2AX foci (B), TRF-1 (C) and telomere dysfunction-induced foci (TIFs, D) in cells were quantified using ImageJ. Data are means ± SD of three replicates. *p<0.05, **p<0.01 vs. LFN. #p<0.05 vs. LFH control.