Figure 3. CDX2 overexpression alleviates hyperglycemia-induced RTECs damage, and CDX2 knockdown aggravates the damage. NRK-52E cells were administered NG (5.5 mM) and HG (25 mM) media for 48h, followed by analysis. (A) Immunofluorescence for E-cadherin and α-SMA detection in NRK-52E cells of the NG and HG groups, respectively (scale bar, 20μm). (B, C) Immunoblot bands of CDX2 (B) and quantitative data (C) in NRK-52E cells of the NG and HG groups. (D–G) Immunoblot bands of E-cadherin, Vimentin, and Col-III (D), and quantitative data (E–G) in NRK-52E cells of the NG and HG groups. H-Q Immunoblot bands of CDX2, E-cadherin, Vimentin, and Col-III in non-transfected (NG or HG treated) NRK-52E cells, and NRK-52E cells transfected with Vector (NG+Vector group, HG+Vector group), or CDX2-overexpressing (NG+OE-CDX2 group, HG+OE-CDX2 group) or CDX2-knockdown (NG+Sh-CDX2 group, HG+Sh-CDX2 group) (H, I); quantitative data are shown (J–Q). Data are mean±SD from three assays performed independently. n=3; *P<0.05 versus NG group; #P<0.05 versus NG+Vector group; &P<0.05 versus HG+Vector group.