Research Paper Volume 13, Issue 5 pp 6804—6819

Transcription factor ELK1 accelerates aerobic glycolysis to enhance osteosarcoma chemoresistance through miR-134/PTBP1 signaling cascade

PTBP1 expression is upregulated in DXR-resistant osteosarcoma tissues and cell lines. (A) PTBP1 expression (normalized to GAPDH) in human osteoblast line, DXR-resistant osteosarcoma cell lines (U2OS/DXR, MG63/DXR, HOS/DXR) and their parental cells determined by RT-qPCR. (B) PTBP1 expression (normalized to GAPDH) in tumor tissues derived from non-responders to DXR treatment (n = 24) than that in tumor tissues derived from responders to DXR treatment (n = 22) determined by RT-qPCR, (C) Overall survival of osteosarcoma patients with high PTBP1 expression, plotted by Kaplan-Meier method. (D) Disease-free survival of osteosarcoma patients with high PTBP1 expression, plotted by Kaplan-Meier method. Values obtained from three independent experiments in triplicate are analyzed by unpaired t test between two groups and by ANOVA followed by Tukey's post hoc test among three or more groups. *p

Figure 2. PTBP1 expression is upregulated in DXR-resistant osteosarcoma tissues and cell lines. (A) PTBP1 expression (normalized to GAPDH) in human osteoblast line, DXR-resistant osteosarcoma cell lines (U2OS/DXR, MG63/DXR, HOS/DXR) and their parental cells determined by RT-qPCR. (B) PTBP1 expression (normalized to GAPDH) in tumor tissues derived from non-responders to DXR treatment (n = 24) than that in tumor tissues derived from responders to DXR treatment (n = 22) determined by RT-qPCR, (C) Overall survival of osteosarcoma patients with high PTBP1 expression, plotted by Kaplan-Meier method. (D) Disease-free survival of osteosarcoma patients with high PTBP1 expression, plotted by Kaplan-Meier method. Values obtained from three independent experiments in triplicate are analyzed by unpaired t test between two groups and by ANOVA followed by Tukey's post hoc test among three or more groups. *p < 0.05 compared with hFOB; *p < 0.05 compared with DXR-resistant osteosarcoma cell lines.