Research Paper Volume 13, Issue 5 pp 6804—6819

Transcription factor ELK1 accelerates aerobic glycolysis to enhance osteosarcoma chemoresistance through miR-134/PTBP1 signaling cascade

PTBP1 promotes chemoresistance of osteosarcoma cells to DXR in vivo. Nude mice were were subcutaneously inoculated with PTBP1-overexpressed or PTBP1-depleted U2OS cells. (A) Tumor volume in nude mice. (B) PTBP1 mRNA level (normalized to GAPDH) in xenograft tumors determined by RT-qPCR. (C) PTBP1 protein level (normalized to GAPDH) in xenograft tumors determined by Western blot analysis. Values obtained from three independent experiments in triplicate are analyzed by unpaired t test. *p

Figure 5. PTBP1 promotes chemoresistance of osteosarcoma cells to DXR in vivo. Nude mice were were subcutaneously inoculated with PTBP1-overexpressed or PTBP1-depleted U2OS cells. (A) Tumor volume in nude mice. (B) PTBP1 mRNA level (normalized to GAPDH) in xenograft tumors determined by RT-qPCR. (C) PTBP1 protein level (normalized to GAPDH) in xenograft tumors determined by Western blot analysis. Values obtained from three independent experiments in triplicate are analyzed by unpaired t test. *p < 0.05 compared with nude mice injected with si-NC-treated U2OS/DXR cells; # p < 0.05 compared with nude mice injected with oe-NC-treated U2OS/DXR cells.