Research Paper Volume 13, Issue 5 pp 6820—6831

Nicardipine sensitizes temozolomide by inhibiting autophagy and promoting cell apoptosis in glioma stem cells

GSCs characteristic analysis and cell survival assay evaluating GSCs sensitivity to TMZ and nicardipine. Under the phase contrast microscope, GSCs showed spherical growth (Scale bar 100μm); CD133, Nestin, and CD44 were positively expressed in GSCs, determined by immunofluorescence staining (Scale bar 50μm) (A). Cell survival assay by CCK-8 after administering TMZ ranging from 0 to 1600 μM, nicardipine (0 to 80 μM), or both for 48 h (B). ** p p

Figure 1. GSCs characteristic analysis and cell survival assay evaluating GSCs sensitivity to TMZ and nicardipine. Under the phase contrast microscope, GSCs showed spherical growth (Scale bar 100μm); CD133, Nestin, and CD44 were positively expressed in GSCs, determined by immunofluorescence staining (Scale bar 50μm) (A). Cell survival assay by CCK-8 after administering TMZ ranging from 0 to 1600 μM, nicardipine (0 to 80 μM), or both for 48 h (B). ** p < 0.01, *** p < 0.001.