Figure 1. GSCs characteristic analysis and cell survival assay evaluating GSCs sensitivity to TMZ and nicardipine. Under the phase contrast microscope, GSCs showed spherical growth (Scale bar 100μm); CD133, Nestin, and CD44 were positively expressed in GSCs, determined by immunofluorescence staining (Scale bar 50μm) (A). Cell survival assay by CCK-8 after administering TMZ ranging from 0 to 1600 μM, nicardipine (0 to 80 μM), or both for 48 h (B). ** p < 0.01, *** p < 0.001.