Research Paper Volume 13, Issue 5 pp 6820—6831

Nicardipine sensitizes temozolomide by inhibiting autophagy and promoting cell apoptosis in glioma stem cells

Detection of autophagy level of GSCs treated with TMZ, CQ, or both. SU4 and SU5 cells were exposed to TMZ (400 μM), CQ (10 μM), or both, respectively for 48 h. Then p62 and LC3 II expression levels were analyzed by Western blotting (A). Statistical analysis of p62 and LC3 II expression in SU4 and SU5 cells (B–E). *p p

Figure 4. Detection of autophagy level of GSCs treated with TMZ, CQ, or both. SU4 and SU5 cells were exposed to TMZ (400 μM), CQ (10 μM), or both, respectively for 48 h. Then p62 and LC3 II expression levels were analyzed by Western blotting (A). Statistical analysis of p62 and LC3 II expression in SU4 and SU5 cells (BE). *p < 0.05, ***p<0.001.