Research Paper Volume 13, Issue 4 pp 6041—6054

Long non-coding RNA CERS6-AS1 facilitates the oncogenicity of pancreatic ductal adenocarcinoma by regulating the microRNA-15a-5p/FGFR1 axis

Downregulation of CERS6-AS1 suppresses PDAC cell proliferation, migration, and invasion and induces cell apoptosis in vitro. (A) CERS6-AS1 expression was detected by RT-qPCR in PANC-1 and SW1990 cells after treatment with si-CERS6-AS1 or si-NC. (B) CCK-8 assays were performed to measure the proliferation of CERS6-AS1-depleted PANC-1 and SW1990 cells. (C) Flow cytometry analysis was used to measure the apoptosis of PANC-1 and SW1990 cells after transfection with si-CERS6-AS1 or si-NC. (D, E) The migration and invasion in PANC-1 and SW1990 cells after CERS6-AS1 silencing was determined with Transwell cell migration and invasion assays. *P

Figure 2. Downregulation of CERS6-AS1 suppresses PDAC cell proliferation, migration, and invasion and induces cell apoptosis in vitro. (A) CERS6-AS1 expression was detected by RT-qPCR in PANC-1 and SW1990 cells after treatment with si-CERS6-AS1 or si-NC. (B) CCK-8 assays were performed to measure the proliferation of CERS6-AS1-depleted PANC-1 and SW1990 cells. (C) Flow cytometry analysis was used to measure the apoptosis of PANC-1 and SW1990 cells after transfection with si-CERS6-AS1 or si-NC. (D, E) The migration and invasion in PANC-1 and SW1990 cells after CERS6-AS1 silencing was determined with Transwell cell migration and invasion assays. *P < 0.05 and **P < 0.01.