Research Paper Volume 13, Issue 4 pp 6041—6054

Long non-coding RNA CERS6-AS1 facilitates the oncogenicity of pancreatic ductal adenocarcinoma by regulating the microRNA-15a-5p/FGFR1 axis

miR-15a-5p plays anti-oncogenic roles and directly targets FGFR1 in PDAC. (A) miR-15a-5p mimic or NC mimic was introduced into PANC-1 and SW1990 cells, and miR-15a-5p expression was measured by RT-qPCR. (B, C) The proliferation and apoptosis of PANC-1 and SW1990 cells following miR-15a-5p overexpression were measured by CCK-8 assays and flow cytometry analysis, respectively. (D) Transwell cell migration and invasion assays were performed to assess the migration and invasion of PANC-1 and SW1990 cells after miR-15a-5p mimic or NC mimic transfection. (E) The miR-15a-5p binding sites in FGFR1. (F) Luciferase activity was detected in PANC-1 and SW1990 cells cotransfected with miR-15a-5p mimic or NC mimic and FGFR1-wt or FGFR1-mut. (G, H) RT-qPCR and western blotting were used to detect FGFR1 mRNA and protein expression in PANC-1 and SW1990 cells after miR-15a-5p overexpression. (I) RT-qPCR confirmation of FGFR1 mRNA expression in paired PDAC tissues and adjacent non-tumor tissues. (J) Pearson’s correlation coefficient was determined to analyze the correlation between FGFR1 mRNA and miR-15a-5p in PDAC tissues. *P

Figure 4. miR-15a-5p plays anti-oncogenic roles and directly targets FGFR1 in PDAC. (A) miR-15a-5p mimic or NC mimic was introduced into PANC-1 and SW1990 cells, and miR-15a-5p expression was measured by RT-qPCR. (B, C) The proliferation and apoptosis of PANC-1 and SW1990 cells following miR-15a-5p overexpression were measured by CCK-8 assays and flow cytometry analysis, respectively. (D) Transwell cell migration and invasion assays were performed to assess the migration and invasion of PANC-1 and SW1990 cells after miR-15a-5p mimic or NC mimic transfection. (E) The miR-15a-5p binding sites in FGFR1. (F) Luciferase activity was detected in PANC-1 and SW1990 cells cotransfected with miR-15a-5p mimic or NC mimic and FGFR1-wt or FGFR1-mut. (G, H) RT-qPCR and western blotting were used to detect FGFR1 mRNA and protein expression in PANC-1 and SW1990 cells after miR-15a-5p overexpression. (I) RT-qPCR confirmation of FGFR1 mRNA expression in paired PDAC tissues and adjacent non-tumor tissues. (J) Pearson’s correlation coefficient was determined to analyze the correlation between FGFR1 mRNA and miR-15a-5p in PDAC tissues. *P < 0.05 and **P < 0.01.