Research Paper Volume 13, Issue 4 pp 6041—6054

Long non-coding RNA CERS6-AS1 facilitates the oncogenicity of pancreatic ductal adenocarcinoma by regulating the microRNA-15a-5p/FGFR1 axis

miR-15a-5p inhibition abolishes the anti-oncogenic actions of CERS6-AS1 knockdown in PDAC cells. (A) RT-qPCR analysis confirmation of miR-15a-5p expression in PANC-1 and SW1990 cells treated with miR-15a-5p inhibitor or NC inhibitor. (B, C) miR-15a-5p inhibitor or NC inhibitor, together with si-CERS6-AS1, was cotransfected into PANC-1 and SW1990 cells. Cell proliferation and apoptosis were detected by CCK-8 assays and flow cytometry analysis, respectively. (D, E) The migration and invasion of the cells described above were measured by Transwell cell migration and invasion assays. *P

Figure 6. miR-15a-5p inhibition abolishes the anti-oncogenic actions of CERS6-AS1 knockdown in PDAC cells. (A) RT-qPCR analysis confirmation of miR-15a-5p expression in PANC-1 and SW1990 cells treated with miR-15a-5p inhibitor or NC inhibitor. (B, C) miR-15a-5p inhibitor or NC inhibitor, together with si-CERS6-AS1, was cotransfected into PANC-1 and SW1990 cells. Cell proliferation and apoptosis were detected by CCK-8 assays and flow cytometry analysis, respectively. (D, E) The migration and invasion of the cells described above were measured by Transwell cell migration and invasion assays. *P < 0.05 and **P < 0.01.