Figure 4. miR-142-3p and SPRED2 bind to each other and miR-142-3p negatively regulates SPRED2. (A) The position of SPRED2 3’ UTR has-miR--142-3p.2 and Luciferase primer pairs of miR-142-3p. (B) Expression of Luciferase Activity in Wild Type and Mutant Type. (C) Dual luciferase report analysis detected the relationship between miR-142-3p and SPRED2. (D) The expression of Spred2 were detected by western blotting and the statistical analysis of western blotting results. *, P < 0.05; **, P < 0.01; ***, P < 0.001.