Research Paper Volume 13, Issue 5 pp 6945—6956

Silencing of miR-483-5p alleviates postmenopausal osteoporosis by targeting SATB2 and PI3K/AKT pathway

miR-483-5p-SATB2 axis regulates the osteogenic differentiation in MC3T3-E1 cells. (A) The transfection efficiency of SATB2 was detected by qRT-PCR. (B) Cell viability was detected 48 h after transfection. (C) LDH release were detected 48 h after transfection. (D) Western blotting results of the indicators of osteogenic differentiation in MC3T3-E1 cells. (E) Quantitative analysis of the optical density in (D). Data were presented as mean ± SD. a p b p

Figure 3. miR-483-5p-SATB2 axis regulates the osteogenic differentiation in MC3T3-E1 cells. (A) The transfection efficiency of SATB2 was detected by qRT-PCR. (B) Cell viability was detected 48 h after transfection. (C) LDH release were detected 48 h after transfection. (D) Western blotting results of the indicators of osteogenic differentiation in MC3T3-E1 cells. (E) Quantitative analysis of the optical density in (D). Data were presented as mean ± SD. a p < 0.05 versus control group, b p < 0.05 versus mimic group.