Figure 3. CCH did not change the neurons density and apoptosis in hippocampus. The brain sections were stained by Nissl staining solutions, NeuN antibody and TUNNEL staining kit. Nissl staining slides were showed (A) (Bar scale= 25μm) and neurons in subregions of hippocampus were counted (B). NeuN labeled neurons were showed (C) (Bar scale= 25μm) and neurons in subregions of hippocampus were counted (D). TUNNEL staining section was showed (E) (Bar scale= 100μm) and counted (F) (n=3 for 4 group).