Research Paper Volume 13, Issue 5 pp 7096—7119

Berberine-induced TFEB deacetylation by SIRT1 promotes autophagy in peritoneal macrophages

Autophagy parameter selection of berberine. (A) Effects of berberine on the viability of peritoneal macrophages. (1) Different concentrations of berberine (100–1000 μmol/L, 6 h). (2) Different concentrations of berberine (50–300 μmol/L, 6 h). (3) Different exposure times to berberine (100 μmol/L). Cell viability was analyzed by CCK-8 assay. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (B) Intracellular accumulation of berberine (100 μmol/L) was detected in peritoneal macrophages (scale bar, 0.1 mm). (C) The expression of LC3 I, LC3 II, and Beclin 1 was analyzed by western blotting after treatment with different concentrations of berberine for 6 h. Quantification of LC3 II/LC3 I ratio and Beclin 1 is shown. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). All values are expressed as mean ± SD (error bars) of three independent experiments. n = 3; *p **p ***p

Figure 1. Autophagy parameter selection of berberine. (A) Effects of berberine on the viability of peritoneal macrophages. (1) Different concentrations of berberine (100–1000 μmol/L, 6 h). (2) Different concentrations of berberine (50–300 μmol/L, 6 h). (3) Different exposure times to berberine (100 μmol/L). Cell viability was analyzed by CCK-8 assay. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (B) Intracellular accumulation of berberine (100 μmol/L) was detected in peritoneal macrophages (scale bar, 0.1 mm). (C) The expression of LC3 I, LC3 II, and Beclin 1 was analyzed by western blotting after treatment with different concentrations of berberine for 6 h. Quantification of LC3 II/LC3 I ratio and Beclin 1 is shown. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). All values are expressed as mean ± SD (error bars) of three independent experiments. n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001 versus control.