Research Paper Volume 13, Issue 5 pp 7096—7119

Berberine-induced TFEB deacetylation by SIRT1 promotes autophagy in peritoneal macrophages

Berberine deacetylates TFEB by activating SIRT1. (A) The mRNA level of SIRT1 following siRNA treatment. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (B) Acetylation levels in primary peritoneal macrophages after different treatments. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (C) Acetylation level of TFEB in the nucleus of primary peritoneal macrophages after different treatments. All values are expressed as mean ± SD (error bars) of three independent experiments. n = 3; *p **p

Figure 8. Berberine deacetylates TFEB by activating SIRT1. (A) The mRNA level of SIRT1 following siRNA treatment. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (B) Acetylation levels in primary peritoneal macrophages after different treatments. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (C) Acetylation level of TFEB in the nucleus of primary peritoneal macrophages after different treatments. All values are expressed as mean ± SD (error bars) of three independent experiments. n = 3; *p < 0.05, **p < 0.01 versus control.