Research Paper Volume 13, Issue 5 pp 7120—7132

Hypoxic pancreatic stellate cell-derived exosomal mirnas promote proliferation and invasion of pancreatic cancer through the PTEN/AKT pathway

Hypoxia upregulates PSC-derived exosomal miR-4465 and miR-616-3p. (A) Heatmap displaying differential expression of plasma exosomal miRNAs between mice implanted with PANC-1 cells alone or combined with PSCs. (B) TEM images illustrating the morphology of exosomes secreted by hypoxic and normoxic PSCs. (C) Western blot detection of exosomal markers in exosomes secreted by hypoxic and normoxic PSCs. (D) Heatmap depicting differential expression of exosomal miRNAs in hypoxic vs normoxic PSCs. (E) Venn diagram showing the overlap between differentially expressed exosomal miRNAs among in vivo and in vitro conditions. (F) RT-qPCR analysis of miR-25-3p, miR-210-3p, miR-4465, miR-652-3p, and miR-616-3p expression in exosomes produced by hypoxic and normoxic PSCs. (G) RT-qPCR analysis of miR-4465 and miR-616-3p expression in plasma exosomes from PC patients (n=50) and healthy controls (n=30). *P

Figure 1. Hypoxia upregulates PSC-derived exosomal miR-4465 and miR-616-3p. (A) Heatmap displaying differential expression of plasma exosomal miRNAs between mice implanted with PANC-1 cells alone or combined with PSCs. (B) TEM images illustrating the morphology of exosomes secreted by hypoxic and normoxic PSCs. (C) Western blot detection of exosomal markers in exosomes secreted by hypoxic and normoxic PSCs. (D) Heatmap depicting differential expression of exosomal miRNAs in hypoxic vs normoxic PSCs. (E) Venn diagram showing the overlap between differentially expressed exosomal miRNAs among in vivo and in vitro conditions. (F) RT-qPCR analysis of miR-25-3p, miR-210-3p, miR-4465, miR-652-3p, and miR-616-3p expression in exosomes produced by hypoxic and normoxic PSCs. (G) RT-qPCR analysis of miR-4465 and miR-616-3p expression in plasma exosomes from PC patients (n=50) and healthy controls (n=30). *P<0.05; **P<0.01.