Research Paper Volume 13, Issue 5 pp 7120—7132

Hypoxic pancreatic stellate cell-derived exosomal mirnas promote proliferation and invasion of pancreatic cancer through the PTEN/AKT pathway

MiR-4465/miR-616-3p inhibition abrogates PSC exosome-induced proliferation, migration, and invasion of PC cells. PANC-1 and MIA PaCa-2 cells were transfected with inhibitors of miR-4465 and/or miR-616-3p, or with the corresponding negative controls (NC), prior to treatment with hypoxic PSC-derived exosomes. (A) Results of CCK-8 proliferation assays. (B) Results of wound-healing migration assays. (C) Results of Transwell invasion assays. *P

Figure 5. MiR-4465/miR-616-3p inhibition abrogates PSC exosome-induced proliferation, migration, and invasion of PC cells. PANC-1 and MIA PaCa-2 cells were transfected with inhibitors of miR-4465 and/or miR-616-3p, or with the corresponding negative controls (NC), prior to treatment with hypoxic PSC-derived exosomes. (A) Results of CCK-8 proliferation assays. (B) Results of wound-healing migration assays. (C) Results of Transwell invasion assays. *P<0.05; **P<0.01.