Research Paper Volume 13, Issue 5 pp 7120—7132

Hypoxic pancreatic stellate cell-derived exosomal mirnas promote proliferation and invasion of pancreatic cancer through the PTEN/AKT pathway

MiR-4465 and miR-616-3p downregulate PTEN and activate AKT in PC cells. (A) Western blot analysis of PTEN, AKT, and p-AKT expression in PANC-1 and MIA PaCa-2 cells transfected with miR-4465, miR-616-3p, or negative control (NC) mimics. (B) Western blot analysis of PTEN, AKT, and p-AKT expression in PANC-1 and MIA PaCa-2 cells transfected with miR-4465 inhibitor, miR-616-3p inhibitor, or the corresponding negative controls (NC) prior to treatment with hypoxic PSC-derived exosomes. *P

Figure 8. MiR-4465 and miR-616-3p downregulate PTEN and activate AKT in PC cells. (A) Western blot analysis of PTEN, AKT, and p-AKT expression in PANC-1 and MIA PaCa-2 cells transfected with miR-4465, miR-616-3p, or negative control (NC) mimics. (B) Western blot analysis of PTEN, AKT, and p-AKT expression in PANC-1 and MIA PaCa-2 cells transfected with miR-4465 inhibitor, miR-616-3p inhibitor, or the corresponding negative controls (NC) prior to treatment with hypoxic PSC-derived exosomes. *P<0.05; **P<0.01.