Research Paper Volume 13, Issue 5 pp 7166—7179

Raddeanin A inhibits proliferation, invasion, migration and promotes apoptosis of cervical cancer cells via regulating miR-224-3p/Slit2/Robo1 signaling pathway

RA decreases the cell viability and inhibits the invasion and migration of CC cells. CCK-8 assay was used to detect the cell viability of (A) Hela and (B) c-33A cells treated with RA. *P*P*PC) Transwell and (D) wound healing assays were respectively conducted for the detection of invasion and migration of Hela cells. (E) Transwell and (F) wound healing assays were employed to examine the invasion and migration of c-33A cells, respectively. (G) Western blot analysis was used to detect the expression of MMP-2 and MMP-9 in both Hela and c-33A cells. *P*P*P#P#P#P

Figure 1. RA decreases the cell viability and inhibits the invasion and migration of CC cells. CCK-8 assay was used to detect the cell viability of (A) Hela and (B) c-33A cells treated with RA. *P<0.05, *P<0.01, *P<0.001 vs. untreated group. (C) Transwell and (D) wound healing assays were respectively conducted for the detection of invasion and migration of Hela cells. (E) Transwell and (F) wound healing assays were employed to examine the invasion and migration of c-33A cells, respectively. (G) Western blot analysis was used to detect the expression of MMP-2 and MMP-9 in both Hela and c-33A cells. *P<0.05, *P<0.01, *P<0.001 vs. control; #P<0.05, #P<0.01, #P<0.001 vs. RA (24 h).