Figure 4. RA activates the Slit2-Robo1 signaling pathway. (A) The expression of Slit2 and Robo1 was detected by western blot analysis in Hela and c-33A cells treated with RA. *P<0.05, *P<0.01, *P<0.001 vs. control; #P<0.05 vs. RA (24 h). (B) The binding sites between miR-224 and Slit2 by bioinformatics analysis. (C) The expression of miR-224-3p after RA treatment was detected by RT-qPCR in these two cell lines. *P<0.01, *P<0.001 vs. control; #P<0.01, #P<0.001 vs. RA (24 h). (D) The expression of miR-224-3p was tested using RT-qPCR after transfection with miR-224-3p mimic in Hela and c-33A cells. *P<0.001 vs. miR-NC. (E) The luciferase reporter assays were used to verify the binding sites between miR-224-3p and Slit2. *P<0.001 vs. miR-NC.