Research Paper Volume 13, Issue 5 pp 7166—7179

Raddeanin A inhibits proliferation, invasion, migration and promotes apoptosis of cervical cancer cells via regulating miR-224-3p/Slit2/Robo1 signaling pathway

Overexpression of miR-224-3p reverses the inhibitory effect of RA on CC cell invasion and migration. The proliferation of (A) Hela and (B) c-33A cells was detected by CCK-8 assay. (C) Transwell and (D) wound healing assays were respectively conducted for the detection of invasion and migration of Hela cells. (E) Transwell and (F) wound healing assays were employed to examine the invasion and migration of c-33A cells, respectively. (G) The expression of MMP-2 and MMP-9 in Hela and c-33A cells was evaluated by western blot analysis. *P#P#P#P

Figure 5. Overexpression of miR-224-3p reverses the inhibitory effect of RA on CC cell invasion and migration. The proliferation of (A) Hela and (B) c-33A cells was detected by CCK-8 assay. (C) Transwell and (D) wound healing assays were respectively conducted for the detection of invasion and migration of Hela cells. (E) Transwell and (F) wound healing assays were employed to examine the invasion and migration of c-33A cells, respectively. (G) The expression of MMP-2 and MMP-9 in Hela and c-33A cells was evaluated by western blot analysis. *P<0.001 vs. control; #P<0.05, #P<0.01, #P<0.001 vs. RA+miR-NC.